Replica Molding and Bonding of Microstructured Hydrogel Plates for Tissue Engineering Applications
نویسندگان
چکیده
We present unique molding/bonding processes to fabricate microstructured Ca-alginate hydrogel plates. We employed propylene glycol alginate (PGA), an ester derivative of alginate, which suppresses the shrinkage of sodium alginate (NaA) during the gelation process, and prepared microstructured hydrogel plates via molding. Also, a bonding procedure utilizing polycation coating is presented to obtain microchannel-incorporating Ca-alginate hydrogel microdevices. We successfully obtained micromachined and bonded hydrogel microfluidic devices, with a bonding property strong enough to introduce an aqueous fluid into the microchannel by pumping. In addition, we formed spheroids/linear aggregates of primary or cultured hepatocytes in the hydrogel microwells of various shapes, by utilizing the non-adhesive surface properties.
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